A natural histone H2A variant lacking the Bub1 phosphorylation site and regulated depletion of centromeric histone CENP-A foster evolvability in Candida albicans.

Eukaryotes have evolved elaborate mechanisms to ensure that chromosomes segregate with high fidelity during mitosis and meiosis, and yet specific aneuploidies can be adaptive during environmental stress. Here, we identify a chromatin-based system required for inducible aneuploidy in a human pathogen. Candida albicans utilizes chromosome missegregation to acquire tolerance to antifungal drugs and for nonmeiotic ploidy reduction after mating. We discovered that the ancestor of C. albicans and 2 related pathogens evolved a variant of histone 2A (H2A) that lacks the conserved phosphorylation site for kinetochore-associated Bub1 kinase, a key regulator of chromosome segregation. Using engineered strains, we show that the relative gene dosage of this variant versus canonical H2A controls the fidelity of chromosome segregation and the rate of acquisition of tolerance to antifungal drugs via aneuploidy. Furthermore, whole-genome chromatin precipitation analysis reveals that Centromere Protein A/ Centromeric Histone H3-like Protein (CENP-A/Cse4), a centromeric histone H3 variant that forms the platform of the eukaryotic kinetochore, is depleted from tetraploid-mating products relative to diploid parents and is virtually eliminated from cells exposed to aneuploidy-promoting cues. We conclude that genetically programmed and environmentally induced changes in chromatin can confer the capacity for enhanced evolvability via chromosome missegregation

Six Months of Multi-Wavelength Follow-up of the Tidal Disruption Candidate ASASSN-14li and Implied TDE Rates from ASAS-SN

We present ground-based and Swift photometric and spectroscopic observations of the candidate tidal disruption event (TDE) ASASSN-14li, found at the center of PGC 043234 ($d\simeq90$ Mpc) by the All-Sky Automated Survey for SuperNovae (ASAS-SN). The source had a peak bolometric luminosity of $L\simeq10^{44}$ ergs s$^{-1}$ and a total integrated energy of $E\simeq7\times10^{50}$ ergs radiated over the $\sim6$ months of observations presented. The UV/optical emission of the source is well-fit by a blackbody with roughly constant temperature of $T\sim35,000$ K, while the luminosity declines by roughly a factor of 16 over this time. The optical/UV luminosity decline is broadly consistent with an exponential decline, $L\propto e^{-t/t_0}$, with $t_0\simeq60$ days. ASASSN-14li also exhibits soft X-ray emission comparable in luminosity to the optical and UV emission but declining at a slower rate, and the X-ray emission now dominates. Spectra of the source show broad Balmer and helium lines in emission as well as strong blue continuum emission at all epochs. We use the discoveries of ASASSN-14li and ASASSN-14ae to estimate the TDE rate implied by ASAS-SN, finding an average rate of $r \simeq 4.1 \times 10^{-5}~{\rm yr}^{-1}$ per galaxy with a 90% confidence interval of $(2.2 - 17.0) \times 10^{-5}~{\rm yr}^{-1}$ per galaxy. ASAS-SN found roughly 1 TDE for every 70 Type Ia supernovae in 2014, a rate that is much higher than that of other surveys.Comment: 21 pages, 9 figures, 6 tables. Photometric data presented in this submission are included as ancillary files. Manuscript updated to reflect changes made in the published version. For a brief video explaining this paper, see https://youtu.be/CTbr-d7cWZ

SN 2016coi (ASASSN-16fp): an energetic H-stripped core-collapse supernova from a massive stellar progenitor with large mass loss

We present comprehensive observations and analysis of the energetic H-stripped SN 2016coi (a.k.a. ASASSN-16fp), spanning the $\gamma$-ray through optical and radio wavelengths, acquired within the first hours to $\sim$420 days post explosion. Our campaign confirms the identification of He in the SN ejecta, which we interpret to be caused by a larger mixing of Ni into the outer ejecta layers. From the modeling of the broad bolometric light curve we derive a large ejecta mass to kinetic energy ratio ($M_{\rm{ej}}\sim 4-7\,\rm{M_{\odot}}$, $E_{\rm{k}}\sim 7-8\times 10^{51}\,\rm{erg}$). The small [\ion{Ca}{ii}] \lam\lam7291,7324 to [\ion{O}{i}] \lam\lam6300,6364 ratio ($\sim$0.2) observed in our late-time optical spectra is suggestive of a large progenitor core mass at the time of collapse. We find that SN 2016coi is a luminous source of X-rays ($L_{X}>10^{39}\,\rm{erg\,s^{-1}}$ in the first $\sim100$ days post explosion) and radio emission ($L_{8.5\,GHz}\sim7\times 10^{27}\,\rm{erg\,s^{-1}Hz^{-1}}$ at peak). These values are in line with those of relativistic SNe (2009bb, 2012ap). However, for SN 2016coi we infer substantial pre-explosion progenitor mass-loss with rate $\dot M \sim (1-2)\times 10^{-4}\,\rm{M_{\odot}yr^{-1}}$ and a sub-relativistic shock velocity $v_{sh}\sim0.15c$, in stark contrast with relativistic SNe and similar to normal SNe. Finally, we find no evidence for a SN-associated shock breakout $\gamma$-ray pulse with energy $E_{\gamma}>2\times 10^{46}\,\rm{erg}$. While we cannot exclude the presence of a companion in a binary system, taken together, our findings are consistent with a massive single star progenitor that experienced large mass loss in the years leading up to core-collapse, but was unable to achieve complete stripping of its outer layers before explosion.Comment: Submitted to ApJ. Main text: 21 pages; Appendix: 15 pages; 12 figure

The Young and Bright Type Ia Supernova ASASSN-14lp: Discovery, Early-Time Observations, First-Light Time, Distance to NGC 4666, and Progenitor Constraints

On 2014 Dec. 9.61, the All-Sky Automated Survey for SuperNovae (ASAS-SN or "Assassin") discovered ASASSN-14lp just $\sim2$ days after first light using a global array of 14-cm diameter telescopes. ASASSN-14lp went on to become a bright supernova ($V = 11.94$ mag), second only to SN 2014J for the year. We present prediscovery photometry (with a detection less than a day after first light) and ultraviolet through near-infrared photometric and spectroscopic data covering the rise and fall of ASASSN-14lp for more than 100 days. We find that ASASSN-14lp had a broad light curve ($\Delta m_{15}(B) = 0.80 \pm 0.05$), a $B$-band maximum at $2457015.82 \pm 0.03$, a rise time of $16.94^{+ 0.11 }_{- 0.10 }$ days, and moderate host--galaxy extinction ($E(B-V)_{\textrm{host}} = 0.33 \pm 0.06$). Using ASASSN-14lp we derive a distance modulus for NGC 4666 of $\mu = 30.8 \pm 0.2$ corresponding to a distance of $14.7 \pm 1.5$ Mpc. However, adding ASASSN-14lp to the calibrating sample of Type Ia supernovae still requires an independent distance to the host galaxy. Finally, using our early-time photometric and spectroscopic observations, we rule out red giant secondaries and, assuming a favorable viewing angle and explosion time, any non-degenerate companion larger than $0.34 R_{\textrm{sun}}$.Comment: 12 pages, 9 figures, 4 tables. Accepted to ApJ. Photometric data presented in this submission are included as an ancillary file. For a brief video explaining this paper, see https://www.youtube.com/watch?v=1bOV-Cqs-a

Discovery and Early Evolution of ASASSN-19bt, the First TDE Detected by TESS

We present the discovery and early evolution of ASASSN-19bt, a tidal disruption event (TDE) discovered by the All-Sky Automated Survey for Supernovae (ASAS-SN) at a distance of $d\simeq115$ Mpc and the first TDE to be detected by TESS. As the TDE is located in the TESS Continuous Viewing Zone, our dataset includes 30-minute cadence observations starting on 2018 July 25, and we precisely measure that the TDE begins to brighten $\sim8.3$ days before its discovery. Our dataset also includes 18 epochs of Swift UVOT and XRT observations, 2 epochs of XMM-Newton observations, 13 spectroscopic observations, and ground data from the Las Cumbres Observatory telescope network, spanning from 32 days before peak through 37 days after peak. ASASSN-19bt thus has the most detailed pre-peak dataset for any TDE. The TESS light curve indicates that the transient began to brighten on 2019 January 21.6 and that for the first 15 days its rise was consistent with a flux $\propto t^2$ power-law model. The optical/UV emission is well-fit by a blackbody SED, and ASASSN-19bt exhibits an early spike in its luminosity and temperature roughly 32 rest-frame days before peak and spanning up to 14 days that has not been seen in other TDEs, possibly because UV observations were not triggered early enough to detect it. It peaked on 2019 March 04.9 at a luminosity of $L\simeq1.3\times10^{44}$ ergs s$^{-1}$ and radiated $E\simeq3.2\times10^{50}$ ergs during the 41-day rise to peak. X-ray observations after peak indicate a softening of the hard X-ray emission prior to peak, reminiscent of the hard/soft states in X-ray binaries.Comment: 23 pages, 14 figures, 5 tables. A machine-readable table containing the host-subtracted photometry presented in this manuscript is included as an ancillary fil

ASASSN-15lh: A Highly Super-Luminous Supernova

We report the discovery of ASASSN-15lh (SN 2015L), which we interpret as the most luminous supernova yet found. At redshift z = 0.2326, ASASSN-15lh reached an absolute magnitude of M_{u,AB} = -23.5+/-0.1 and bolometric luminosity L_bol = (2.2+/-0.2)x 10^45 ergs s^-1, which is more than twice as luminous as any previously known supernova. It has several major features characteristic of the hydrogen-poor super-luminous supernovae (SLSNe-I), whose energy sources and progenitors are currently poorly understood. In contrast to most previously known SLSNe-I that reside in star-forming dwarf galaxies, ASASSN-15lh appears to be hosted by a luminous galaxy (M_K ~ -25.5) with little star formation. In the 4 months since first detection, ASASSN-15lh radiated (1.1+/- 0.2)x10^52 ergs, challenging the magnetar model for its engine.Comment: Published in the January 15, 2016 Issue of Science Magazin

ASASSN-18di: discovery of a ΔV∼10ΔV \sim 10 flare on a mid-M dwarf

We report and characterize a white-light superflare on a previously undiscovered M dwarf detected by the ASAS-SN survey. Employing various color-magnitude and color-spectral type relationships, we estimate several stellar parameters, including the quiescent V-band magnitude, from which we derive a flare amplitude of $\Delta V \sim 10$. We determine an r-band absolute magnitude of $M_{r} = 11.4$, consistent with a mid-M dwarf, and an approximate distance to the source of $2.2$ kpc. Using classical-flare models, we infer a flare energy of $E_{V} \simeq (4.1\pm 2.2)\times 10^{36}$ ergs, making this one of the strongest flares documented on an M dwarf